Rapid Cycle Real-Time PCR — Methods and Applications

for Microbiology Volume of Rapid Cycle Real-Time PCR.- Applications and Challenges of Real-Time PCR for the Clinical Microbiology Laboratory.- I Bacteriology.- Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimens by LightCycler PCR.- Rapid Detection and Simultaneous Differentiation of Legionella spp. and L. pneumophila in Potable Water Samples and Respiratory Specimens by LightCycler PCR.- Homogeneous Assays for the Rapid PCR detection of Burkholderia pseudomallei 16s rRNA gene on a Real-Time Fluorometric Capillary Thermocycler.- Rapid Detection of Toxigenic Corynebacterium diphtheriae by LightCycler PCR.- Rapid Detection of Resistance Associated Mutations in Mycobacterium tuberculosis by LightCycler PCR.- Duplex LightCycler PCR Assay for the Rapid Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Species Confirmation.- Rapid Detection of Group B Streptococci Using the LightCycler Instrument.- Rapid Detection and Quantification of Chlamydia trachomatis in Clinical Specimens by LightCycler PCR.- Quantification of Toxoplasma gondii in Amniotic Fluid by Rapid Cycle Real-Time PCR.- Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto.- Rapid and Specific Detection of Coxiella burnetii by LightCycler PCR.- Molecular Identification of Campylobacter coli and Campylobacter jejuni Using Automated DNA Extraction and Real-Time PCR with Species-Specific TaqMan Probes.- Rapid and Specific Detection of Plesiomonas shigelloides Directly from Stool by LightCycler PCR.- Quantification of Thermostable Direct Hemolysin-Producing Vibrio parahaemolyticus from Foods Assumed to Cause Food Poisoning Using the LightCycler Instrument.- II Detection of Fungal Pathogens.- Quantification and Speciation of Fungal DNA in Clinical Specimens Using the LightCycler Instrument.- III Virology.- Development, Implementation, and Optimization of LightCycler PCR Assays for Detection of Herpes Simplex Virus and Varicella-Zoster Virus from Clinical Specimens.- Qualitative Detection of Herpes Simplex Virus DNA in the Routine Diagnostic Laboratory.- Use of Real-Time PCR to Monitor Human Herpesvirus 6 (HHV-6) Reactivation.- Simultaneous Identification of Five HCV Genotypes in a Single Reaction.- Rapid Detection of West Nile Virus.- Use of Rapid Cycle Real-Time PCR for the Detection of Rabies Virus.- Development of One-Step RT-PCR for the Detection of an RNA Virus, Dengue, on the Capillary Real-Time Thermocycler.- IV Detection of Genetically Modified Organisms (GMO).- Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase).