Bert, van Duijn,
Anneke Wiltink
Signal Transduction — Single Cell Techniques
Paperback Engels 2012 1998e druk 9783642489761Levertijd ± 9 werkdagen
Gratis verzonden
Samenvatting
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
Specificaties
ISBN13:9783642489761
Taal:Engels
Bindwijze:Paperback
Aantal pagina's:468
Uitgever:Springer Berlin Heidelberg
Druk:1998
Serie:Springer Lab Manuals
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