Gel Chromatography

Gel Filtration · Gel Permeation · Molecular Sieves A Laboratory Handbook

Paperback Engels 1969 2e druk 9783642495953
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Samenvatting

The efforts spent on many a scientific book cannot be justified, no matter how many words are said about it. The opposite is true for this book and a few brief remarks upon its publication. Within a short period of time, short even by all present standards, gel chromatography has gone through a development and experienced an accep­ tance that are unknown to any other method. From experience the new and unique separation technique is today known and liked in all laboratories that are concerned with substances of high molecular weight; in others, the technique is known from hearsay, at least. It has become evident that a comprehensive coverage of the conceptual development, the theoretical principles, and the experimental technique of the new method would be desirable. This coverage is now offered by the book of an expert. Its author has personally participated in the development from its beginning and has helped to promote it. He initiated gel chromatography of many substances, including proteins, on thin-layer plates; for lipophilic substances he has contributed considerably to the transition from water to organic solvent systems and developed theoretical concepts for a better understanding of the effects that are responsible for the separation. This book appears to me to point in new directions. The reader receives not only a clear presentation of facts but also instructions for practical applications.

Specificaties

ISBN13:9783642495953
Taal:Engels
Bindwijze:paperback
Aantal pagina's:202
Uitgever:Springer Berlin Heidelberg
Druk:2

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Inhoudsopgave

1 Introduction.- Separation by Molecular Weight Differences.- Dialysis.- Homogeneous Gels.- Chromatography on Granulated Gels.- Illustrative Experiment.- Terminology.- Historical Survey.- Ion Exchangers.- Neutral Gels.- References.- 2 Materials and Methods.- The Gels.- Preparation of Hydrophilic Gels.- Dextran Gels.- Polyacrylamide Gels.- Agar Gels, Agarose Gels.- Preparation of Organophilic Gels.- Sephadex Derivatives.- Acrylic Gels.- Polystyrene Gels.- Aerogels.- Commercial Gels.- Sephadex.- Sephadex LH-20.- Bio-Gel.- Agarose.- Styragel.- Equipment.- Columns.- Pumps.- Hose Connections.- Detectors.- Fraction Collectors.- Integrated Units.- Experimental Techniques with Columns.- Packing of the Gel Bed.- Chromatography.- Sample Application.- Elution.- Analysis.- Extension of the Gel Bed.- Recycling Chromatography.- Thin-layer Gel Chromatography.- Preparation.- Chromatography.- Results.- Centrifuge Technique.- Equipment.- Illustrative Experiments.- Desalting.- Concentrating.- References.- 3 Theory.- Numerical Evaluation of Experimental Data.- Volumes in the Gel Bed.- Elution Parameters.- Efficiency of a Gel Column.- Methods of Interpretation.- The Exclusion Principle.- Simple Geometric Models.- The Ogston-Laurent Gel Model.- The Principle of Restricted Diffusion.- Comparison of the Various Interpretations.- The Partition Principle.- Affinity of Solutes for the Gel Phase.- Partition and Adsorption.- Hydrophilic Gels.- References.- 4 Principles of Application.- Gel Filtration.- Desalting.- Group Separation.- Modification of Macromolecules.- Complex Formation.- Gel Chromatography.- Molecular Weight Determination.- Calibration.- Technique.- Molecular Weight Determination of Proteins.- Theory.- Results.- Association Equilibria.- The Study of Polydisperse Systems.- General Principles.- Gel Chromatography.- Evaluation of Gel Permeation Chromatograms.- Results.- Separation without Differences in Size.- Chromatography in Aqueous Media.- Chromatography with Organic Solvents.- Fractional Precipitation.- References.- 5 Results.- Enzymology.- Isolation of Enzymes.- Enzymatic Reactions.- Endocrinology.- Chemistry of Plasma Proteins.- Structure Elucidation of Proteins.- Nucleic Acid Chemistry.- Nucleic Acids.- Oligonucleotides.- Virus Separation.- Carbohydrates.- Clinical Chemistry.- Miscellaneous Applications.- References.- Author Index.

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        Gel Chromatography