Cell-Free Protein Expression

Nemetz Generation of Linear Expression Elements by PCR Nemetz et al. Rapid Protein Engineering by Expression-PCR Stiege Expression-PCR: From Gene Pools to Purified Proteins within One Day Watzele High-throughput expression PCR Used to Systematically Investigate Regulation of Translation Initiation in an E. coli Cell-Free Expression System Gräntzdörffer Reduction of Primer-Dimer Formation During Generation of Expression Fragments by PCR Pelton Isotope Labeling of Proteins for NMR Spectroscopy Using Cell-Free Methods Fernholz Production of a Specifically Labeled Protein in mg Quantities for NMR Analysis Schlößmann In Situ Mono-Biotinylation of Cell-Free Expressed Proteins Using the AviTag Technology Schräml Rapid Generation of Protein Variants and Subsequent Analysis by Surface Plasmon Resonance Bernhard Incorporation of Fluorescence Labels into Cell-Free Produced Proteins Budisa Expression of 'tailor-made' proteins via Incorporation of Synthetic Amino Acids by Using Cell-Free Protein Synthesis Beernink Application of Cell-Free Expression Systems to Proteomic Studies Busso/Kim Screening and Increasing Soluble Expression of Recombinant Proteins using the RTS 100 HY in a 96-Well format Yamane In vitro expression of proteins with disulfide bridges and ist application for high-throughput screening system Kim Cell-free expression of proteins containing multiple disulfide bonds Maurer Cell-free Synthesis of Membrane Proteins on a Preparative Scale Betton Using Maltose-Binding Protein Fragment Complementation to Probe Protein-Protein Interactions by Co-Expression in the RTS System Lorenz In-vitro translation of KRAB Zinc Finger Transcriptional Repressor Proteins and their Interaction withtheir TIF1ß Co-Repressor Lehmann Optimization of Cell-Free Expression of FAD-dependent D-Amino Acid Oxidase Liu Expression of Recombinant Chemokine-like Factor 1 with a Cell-Free Protein Biosynthesis System Bosserhoff Recombinant Expression of Functional Active MIA Protein for Mutation Analysis Using RTS System Bochtler-Hock Cell-Free Expression of the Heterodimeric Protein Penicillin G Amidase in a Functional Active Form Bittorf Cell-Free Expression of the His-Tagged Recombinant Prolactin-like Placenta Protein E Using the RTS 500 System Iskakov Complementary Interaction Between the Central Domain of 18S rRNA and the 5´Untranslated Region of mRNA Enhances Translation Efficiency in Plants Kubick In vitro translation in an insect-based cell-free system