Rapid Cycle Real-Time PCR — Methods and Applications

for Genetics and Oncology Volume Rapid Cycle Real-Time PCR Methods and Applications.- I Methods Useful in Genetics and Oncology.- Quantification of Cytokine mRNAs in Human Myocardial Biopsy Samples by Real-Time Quantitative PCR Technology Using the LightCycler Instrument.- Quantitative Two-Step RT-PCR for the Detection of Human ABCA1 Transporter on LightCycler Using Hybridization Probes and External Standards.- Quantification of Human Genomic DNA Using Retinoic X Receptor B Gene.- Genotyping by Guanosine-Dependent Quenching of Single-Labeled Fluorescein Probes.- Limitations of Melting Curve Analysis Using SYBR Green I — Fragment Differentiation and Mutation Detection in the CFTR-Gene.- SYBR Green I Analysis of the Trinucleotide Repeat Responsible for Huntington’s Disease.- II Applications in Genetics.- Parallel Genotyping of Different Genes: A Rapid Real-Time PCR Approach.- Detection of a Single Base Substitution in Single Cells by Melting Peak Analysis Using Dual-Color Hybridization Probes.- Rapid Screening for Five Major Cystic Fibrosis Mutations by Melting Peak Analysis Using Fluorogenic Hybridization Probes.- LightCycler PCR for the Polymorphisms —308 and —238 in the TNF AIpha Gene and for the TNFB1/B2 Polymorphism in the LT Alpha Gene.- Rapid Genotyping of 2-bp and 9-bp Deletion Mutations Using the LightCycler.- Genotyping of the Methionine-Valine Polymorphism at Codon 129 of the Human Prion Protein by Melting Point Analysis of Fluorescently Labeled Hybridization Probes.- Rapid Detection of Missense Mutations in the Prostatic Steroid 5?-Reductase Gene Using Real-Time Fluorescence PCR and Melting Curve Analysis.- III Applications in Oncolon.- Analysis of Microsatellite Instability by Melting Peak Analysis with BAT26 and BAT25 Specific Fluorescence Hybridization Probes.- Two Color Multiplexing and Typing of Human Papillomavirus Types 16,18 and 45 on LightCycler.- Quantitative Analysis of AML1-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR.- Rapid Quantitative Detection of Free Cancer Cells in the Peritoneal Cavity of Gastric Cancer Patients with Real-Time CEA RT-PCR Using Hybridization Probes.- Quantitative Measurement of the mRNA Expression of the Tumor-Associated Antigen PRAME by Real-Time RT-PCR Using LightCycler and SYBR Green I Technology.- Expression Analysis of Telomerase-Genes hTERT and hTR by Quantitative PCR on LightCycler.- Measurement of MDR1 Gene Expression by Real-Time Quantitative RT-PCR Using the LightCycler Instrument.