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Antibody Usage in the Lab

Paperback Engels 2012 1999e druk 9783662039441
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A handy lab manual that permits quick and easy access to the techniques commonly used to analyse antibody specificity. Some of the most useful immunological techniques based on antibodies are described, including ELISA, immunoblotting and immunoprecipitation protocols that provide useful tools for recognising immunological specificities, and basic immunofluorescence and immunohistochemistry procedures for the in situ identification of antigens. The topics are discussed from a practical point of view, describing the theoretical basis of each technique and sample protocols plus a troubleshooting guide. Attention is focused on the various aspects of the protocols described thus providing the reader with the maximum possible information on each technique.

Specificaties

ISBN13:9783662039441
Taal:Engels
Bindwijze:paperback
Aantal pagina's:154
Uitgever:Springer Berlin Heidelberg
Druk:1999

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Inhoudsopgave

1 Introduction.- 2 ELISA assays.- 3 Immunoblotting.- 4 Immunoprecipitation.- 5 Immunofluorescence.- 6 Immunohistochemistry.- 7 Antibody Specificity Analyzed by Peptides Synthesized on Cellulose Membranes.- Appendix 1.- Appendix 2.- Appendix 3.

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