Biochemical Engineering for 2001

1. Overview.- Biochemical Engineering — Past, Present and Future.- Strategic Planning and New Directions in Biochemical Engineering.- New Biocatalytic Functions of Microorganisms and Their Industrial Applications.- 2. Gene and Protein Engineering.- Development of a Bacillus brevis Host-Vector System for Efficient Heterologous Protein Production and Its Application to Protein Engineering.- Chimeric Receptor Made from IgM and EGFR that Induces Phosphorylation Upon Binding with Antigen.- Molecular Cloning of Feline Interferon cDNA by Direct Expression and Its Mass Production Using Silkworm.- Expression of Human Calcitonin in Escherichia coli.- Control of Gene Expression and Overproduction of Useful Heterologous Protein by Using an On-Line Sensor.- Expression and Secretion of Cloned Proteins in Recombinant Saccharomyces cerevisiae.- Plasmid Loss Probability in Escherichia coli as a Function of the Growth Rate of Plasmid-Bearing Cells. I: Variation During Continuous Cultures.- Plasmid Loss Probability in Escherichia coli as a Function of the Growth Rate of Plasmid-Bearing Cells. II: Modelling for Continuous Cultures.- Cloning and Expression of the Xylose (Glucose) Isomerase Gene from the Thermophile Thermus thermophilus HB8.- Mutagenesis Studies on the Amino Acid Residues Involved in an Iron-Binding Domain and a Catalytic Site of Human 5-Lipoxygenase.- Technology of Production of Recombinant Prourokinase.- High Level Expression and Secretion of Biologically Active Leech Hirudin Variant 1 (HV1) by Escherichia coli.- Transfer of Isolated Nuclei of Saccharomyces cerevisiae Into Anucleated Protoplasts of Nectaromyces species.- 3. Enzyme Engineering.- In Vitro Protein Refolding.- Nitrile Hydratase: Investigation of Its Light-Induced Activation.- Cloning of Bacillus licheniformis Amylase Gene and Its Application to Synthesis of Branched Oligosaccharides.- Aldolases for Use in Carbohydrate Synthesis : Enzymatic Reaction Engineering as a Tool for Process Optimization.- Bioconversion of Gaseous Substrate by Methylotrophic Hansenula polymorpha in a Gas-Solid Bioreactor.- Asymmetric Hydrolysis of Phenylglycidate Ester by Esterase from Serratia marcescens.- D-Threonine Aldolase and Its Application to D-ß-Hydroxy-a-Amino Acid Synthesis.- Improvement of Enzymatic Properties by Hydrophobic Interaction.- The Effect of Amphopathics on the Properties of Enzymes (Lipase, Cholinesterase, Cellulase).- Modified Cellulase with Amphiphilic Copolymers: Solubilization in Organic Solvent.- Aminoacylase-Catalyzed Synthesis of N-Acyl Amino Acid from Fatty Acid or Its Ethyl Ester and Amino Acid.- Peptide Synthesis in Organic Solvent with an Immobilized Enzyme.- A Novel Interesterification Process for Fats and Oils Using Acetone-Dried Fungus Immobilized in Biomass Support Particles.- Fractionation of Cellulase Components by Affinity Precipitation: Production of Cellobiose by Enzymatic Hydrolysis.- Separation of Positional Isomers by Combined Use of Enzymatic Hydrolysis and Extraction.- Studies on the Synthesis and Properties of the Mimics with Glutathione Peroxidase Activity and Preparation of the Conjugate of Mimic-Antibody.- Direct Synthesis of Cyclodextrin in a Heterogeneous Enzyme Reaction System Containing Insoluble Extruded Starch.- 4. Fermentation Technology.- Engineering and Genetic Approaches to Optimization of Recombinant Fermentation Process.- Computer-Controlled Fed-Batch Cultivation of a Recombinant Escherichia coli for Overproduction of Human Interleukin-2.- Production of Recombinant HTLV-I Envelope Protein.- Computer-Controlled High Cell Density Cultivation of Recombinant Escherichia coli Producing ß-Galactosidase.- Use of an Adaptive Control Strategy for the Production of Exotoxin A from High Density Culture of Recombinant Escherichia coli.- Effective Extracellular Production of Heterologous Proteins by Automated Fed-Batch Cultures of Recombinant Microorganisms Carrying Excretion Plasmid Vectors.- Production of Antifungal Peptide Produced by Bacillus subtilis in Solid State Fermentation.- Dynamics of Trichoderma reesei Pellet Formation and Oxygen Penetration Phenomenon in the Pellet.- Studies on Drying Pullulan by Using Spray-Fluid-Bed with Carriers Circulation.- Molecular Breeding of a Biotin-Hyperproducing Strain of Serratia marcescens.- Hyper-Production of Thermostable Lipase by Genetically Engineered Pseudomonas Species.- Effects of In Vitro Protein Stabilizers on the Overproduction of ß-Lactamase in Escherichia coli.- Fermentation Conditions for Maximizing Hemolysin Production in Batch and Continuous Cultures of Recombinant Escherichia coli.- Reconsideration of Commercially Available Medium Component in Recombinant Yeast Culture.- Production of Hepatitis B Surface Antigen Including PreS(PreS-HBsAg) in Fed-Batch Culture of Recombinant Yeast.- Growth and Induction Effects on the Expression of rhTNF-ß.- Production of Thermophilic Protease (Aqualysin I) by Fed-Batch Cultures of Recombinant Escherichia coli.- Production of Poly-3-Hydroxybutyric Acid (PHB) by Alcaligenes eutrophus H16 in a Fed-Batch Culture.- Kinetic Analysis of Continuous and Batch Cultures with Streptococcus faecalis.- Kinetic Study of Lysine Fermentation in Cane Molasses Base Medium.- A New Scale-Up Method Based on the Effect of Ventilation on Aerated Fermentation Systems.- Measurement of Shaking-Flask Performance and Its Application to Scaling Up a Fermentation Process to a Stirred-Tank Fermentor.- Effect of Magnesium Sulfate on the Feedback Inhibition of Sisomicin Production.- Effect of pH and Its Optimization for High Molecular Weight of Pullulan.- Production of Biosurfactants by Candida Antarctica.- Photometric Measurement of High Cell Density Culture Broth with Continuous Dilution System.- Continuous and High Cell Density Cultivation of Escherichia coli W3110 (pEC901) Harboring Human Interferon Alpha A Gene.- Purification and Characterization of Nitrilase Catalyzing Asymmetric Hydrolysis of Aminonitriles from Rhodococcus rhodochrous PA-34.- Carbon Mass Balance Analysis of Sophorose Lipid Production by Torulopsis bombicola.- Gel Formation by Methylan Produced from Methanol.- 5. Plant Cell Culture.- Extractive Plant Cell Culture.- Studies on the Production of Secondary Metabolites in Plant Cell Culture.- Studies on Production of Flavonol Glycosides in Cell Cultures of Ginkgo biloba.- Flavonoid Production from a Cell Suspension Culture of Scutellaria baicalensis G.- Increasing Secondary Metabolite Production by In Vivo Extraction and Elicitation.- Investigation of the Production of Shikonin Derivatives from Immobilized Lithospermum erythrorhizon Cells by Intermittent Permeabilization.- Production of Red Pigments by Perilla frutescens Cells in Bioreactors.- Production of Plant Secondary Metabolites from High Density Perfusion Cultures.- Production of Artificial Seeds.- ß-Carotene Production by DO-Stat Semi-Continuous Culture of Carrot Cells in an Air-Lift Column with Draft Tube.- Effects of Bioregulators and Administration Methods on the Biotransformation of Monoterpenes by Cultured Cells of Eucalyptus perriniana.- Production of Cardenolides by Plant Cell and Tissue Cultures.- Effects of Rate Limiting Factors on Vitamin E Production Using Safflower Cells.- Stimulation of Shikonin Derivative Accumulation in Onosma paniculatum and Lithospermum erythrorhizon Cell Culture by Fungal Elicitors.- Production of Red Pigment by Tissue Culture of Phytolacca americana.- Production of Essential Oil by Mentha piperita Cell Culture.- Bioreactor-Based Culture of Plant Hairy Roots for Production and Recovery of Pigments.- Caffeine Production by Polyurethane Foam Immobilized Coffee (Coffea arabica L.) Cells.- Design of Tubular Microporous Membrane Aerated Bioreactors for Plant Cell Cultures.- 6. Animal Cell Culture.- Proteins and Pesticides from the Insect Cell-Baculovirus System.- Study of Hybridoma Cells, Kinetics in High Density Culture.- Physiological Modelling of Uptake and Partitioning of Glucose and Glutamine for Growth and Antibody Production by a Mouse Hybridoma.- The Effects of Glutamine and Glucose Concentration on Hybridoma Cell Growth and Antibody Productivity.- Development of Large-Scale Suspension Reactor for Anchorage-Dependent and Independent Mammalian Cell Culture.- Production of t-PA by Recombinant Mouse Fibroblast Cell Cultures in a Perfusion Bioreactor.- Microcarrier Culture of Fish Cells and Viruses in Cell Culture Bioreactor.- Application of Three Dimensional Culture of Adult Rat Hepatocytes in PUF Pores for Artificial Liver Support System.- Effects of Glucose and Lactate in Insect Cell (Sf9) Culture.- Large-Scale Culture of Vero Cells on GT-2 Microcarrier in Cell Culture Bioreactors.- Growth of Anchorage-Independent Animal Cells Captured in Newly Developed Porous Microcarriers.- A Packed-Bed Bioreactor Utilizing Reticulated Polyvinyl Formal Resin to Attain High Density Culture of Hepatocytes.- High Density Culture of Hybridoma Cell Using Perfluorocarbon for Oxygen Supply.- Suspension Culture of Insect Cells in a Spin-Filter Bioreactor for Recombinant Protein Production.- A Selective Ammonia Removal System for Hybridoma Cell Cultures.- High Density and Stable Immobilization of Floating Spheroids of Rat Hepatocytes for an Artificial Liver Module.- Generation and Characterization of Anti-Peptide Monoclonal Antibodies Recognizing Transforming Growth Factor ß-1.- Effect of Nutrients and Metabolites on Hybridoma Cell Growth and Metabolism.- 7. Bioreactor.- Role of the Reactor in Optimizing Productivity of Suspension Culture Animal Cells.- Autotroph Culture of Alcaligenes eutrophus Using H2, O2 , and CO2 Gas-Mixture Adjusting out of Explosive Region.- Production of Sorbitol with Cetyltrimethylammoniumbromide Permeabilized cells of Zymomonas mobilis.- Ethanol Fermentation in the Liquid-Solid Downward Flow Light Particle Fluidized Bed Bioreactor.- Fermentation of Banana Wine with Immobilized Yeast.- The Bioconversion of Sugar Beet Pulp into Microbial Protein with Aspergillus tamarii by Solid State Fermentation.- Production of Alkaline Protease by Semi-Solid-State Fermentation Using Urethane Foam as Carrier.- Microcarrier Motion and Its Effect on Mass Transfer Inside a Horizontally Rotating Bioreactor for Animal Cell Culture.- Fed-Batch Culture in an Airlift Reactor with a Net Draft Tube.- Characterization of Liquid-Impelled Loop Reactors.- An Investigation of Mass Transfer Characteristics of a Dynamic Bioreactor.- Modelling of a Bioreactor: Optimization of a Loop Reactor for Biochemical Systems.- Studies on the Performance of Bioreactors Used for the Fermentation Process with Highly Viscous Broth.- Electro-Ultra Filtration Bioreactor for Enzymatic Reaction in Reverse Micelles.- The Application of Airlift Reactors for Immobilized Enzyme Reactions.- Vinegar Production in a Bioreactor with Chitosan Beads as Supports of Immobilized Bacteria.- Continuous Production of Biomass and Metabolites in High Cell Density Membrane Bioreactor.- Investigation into the Immobilization of Yeast Cell by Alginate Gel Beads.- Kinetics of Gel Swelling in Pure Water and Collapse in an Electric Field.- A Study on the Development of an Annulus-Airlift Fermentor for Production of 2-Keto-L-Gulonic Acid.- Investigation of the Characteristics of an Air-Lift Loop Reactor with Internal Circulation and Its Use for Glutamic Acid Fermentation.- Hydrodynamics and Mass Transfer Characteristics of Mycelial Fermentation Broths in a Novel Bubble Column.- Continuous Synthesis of Oligopeptide by Free Enzyme in Pulsed Extraction Column Bioreactor.- Enzymatic NAD+Recycling in Large Liposomes Prepared by Means of the Two-Step Emulsification Procedure.- pH Profiles in Biochemical Reactors.- Continuous Operation of Olive Oil Hydrolysis with Lipase in “Vibro Mixer”.- Biochemical Production by Living Cell Reaction Processes.- Parameteric Analysis of Effect of Oxygen Tension on Citric Acid Production by Surface Culture.- Disruption of Yeast and Partitioning of Proteins and Cell Debris in Aqueous Two-Phase Systems.- Steam Control and Oxygen Solubilization Using a Cross Flow Fan in an Agitating Bioreactor.- 8. Bioseparation.- Recent Advances in Bioseparation.- Scaling Rules of Nonlinear, Coupled Elution Chromatography.- Preparative HPLC of Macrolide Antibiotics.- Separation of Hyaluronic Acid from Plant and Animal Tissues.- Immunorecognition of Anti-Peptide Antibodies and Application to Separation of Biomolecules.- Towards Enhanced Specificity During the Purification of Microbial Proteins.- Protein Separation in the Medium of Organic Solvent by High Electrical Potential.- Concentration of Tocopherols from Soybean Sludge by Supercritical Carbon Dioxide.- Mass Transfer Rate and Simulation of Supercritical Fluid Extraction of Bioproducts.- Crossflow Membrane Filtration of Highly Viscous Microbial Broth.- Functionalized Membranes for Adsorption of Proteins.- Fractionation of Escherichia coli on the Rupture of Bubbles at a Liquid Surface.- Separation of Proteins by New Reversed Micelles.- Mass Transfer Rate in Protein Extraction by Reverse Micelles.- Predicting the Performance of Production-Scale Chromatography.- Separation and Purification of Bilirubin from Hog Bile.- Affinity Purification of Proteins Using Colloidal Particles.- Metal Affinity Partitioning of Phosphoproteins in Aqueous Two-Phase Systems.- Partition Coefficients of Fatty Acids Between Water and n-Hexane.- Chromatographic Behavior of Lipids with Supercritical Fluid Mobile Phase.- Extraction of Indole Alkaloids from Catharanthus roseus Using Supercritical Carbon Dioxide.- Determination of Elution Conditions of Immunoaffinity Chromatography by ELISA (Enzyme Linked Immunosorbant Assay) Method.- Separation and Concentration of Ampholytes by Recycling Free Flow Electrophoresis.- Bioproduct Adsorption on Encapsulated Adsorbent: Local Thermodynamic Equilibrium Model.- Shear Breakage of Bacterial Cells With Crossflow Filtration.- Extraction of Trehalose from Baker’s Yeast.- Activity and Conformation of Lysozyme in Reverse Micellar Extraction Processes.- Investigation on Large Scale Production of Steroid Drugs From Agave Plants.- The Effect of Amino Acid Sequence on the Separation of Peptides in an Aqueous Two-Phase System.- Separation of Butanol from the Extraction Fermentation Broth by Pervaporation.- Application of Pervaporation with Supported Liquid Membrane to Volatile Fermentation.- Membrane Affinity Chromatography Using Hollow Fibers Containing Hydrophobic Amino Acids as a Ligand.- Molecular Design and Construction of Affinity Ligands Using Engineered Enzymes.- 9. Biosensor and Biomedical Engineering.- Electrically Controlled Cell Culture and Its Biomedical Applications.- Development of a Delivery System for Carboxylic Ionophores: A Potentiator of Immunotoxin Toxicity Using Liposome as Carrier.- Function of Immobilized Heptocytes and Their Application for Artificial Liver Support.- Development of a Novel EN-FET as Pesticide Monitor.- A New Method for Analyzing Bacterial Abilities to Sense and Respond to Chemical Stimuli.- Development of Noninvasive Glucose Sensor by Electrogenerated Chemiluminescence for Clinical Applications.- Binding of Affinity Ligand with a Three-Dimensional Target: The Role of Diffusion Limitations.- Fiber Optic Biosensors: Potential Designs for Fermentation Sensing.- Liposome-Based Immunoassay for Complement Activity and Its Application to Clinical Tests.- Biospecific Antibodies for Thromolysis.- Antifibrosis Effect of Enzyme Compositions (Superoxide Dismutase, Catalase, Hyaluronidase) in Experimental Silicosis.- 10. Process Modelling and Control.- Structured Models for Growth in Bioreactors with External Sources of Biomass.- Parameter Estimation in Fermentation Using Partial Data.- A Rapid Method of Parameter Estimation for a Heterogeneous Model of Protein Adsorption in Stirred and Recirculation Vessels.- Growth Controlled Fed-Batch Cultivations: Theory, Development of Control Strategies, and Experimental Verification.- Maximizing Lysine Production in Fed-Batch Culture by Controlling the Specific Growth Rate Under Leucine-Limited Conditions.- MIMO Adaptive Control for Fed-Batch Penicillin Fermentation.- Control of a Fed-Batch Bioreactor Using Learning Automata.- Knowledge-Based Diagnosis of Inoculum Properties in Lactic Acid Fermentation 681.- Bifurcation and Chaos in an Enzymatic Membrane System.- Advanced Control of pH in Mammalian Cell Culture.- Dissolved Oxygen Concentration and Growth Rate Control Using Auto-Tuning PID Controller in Fed-Batch Fermentation Process.- Learning Control of a Fed-Batch Fermentation with Substrate Inhibition Kinetics.- Modelling and Adaptive State Estimation for Fed-Batch Fermentation.- Simulation of Switching Phenomena in Biological Systems.- On-Line Monitoring of Bioreactors by Fluorometry.- 11. Bioresources and Food Engineering.- Application of a Cellulase Enzyme System and an Integrated Process for Biomass Conversion into Ethanol.- Biomass Production from Carbon Dioxide by a Marine Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus.- to CMA Applications.- Mathematical Model for Degradation of Mixed Substrates by Immobilized Cells.- Development of Shielded Hot-Wire Viscosity Sensor for Flowing Systems and Its Application to the Monitoring of Xanthan Gum Fermentation.- Pasteurization by Far Infrared Irradiation Below Lethal Temperature of Bacteria.- Preparation of a Peptide Mixture with Nutritionally Definite Characteristics from a Protein Hydolysate.- Direct Alcohol Fermentation of Starch by Schwanniomyces castellii.- Alcohol Fermentation of Mixed Sugars Contained in Lignocellulose Material by Pichia stipites.- The Pretreatment and Separation of the Biomass Component for Total Utilization.- A Solution of the Theoretical Yield of Bio-Metabolic Products by Thermodynamic Method.- Biological Control of Bacterial Wilt Disease.- A Proposed Design Method for Predicting Cleaning Parameters of the Bio-Plant.- Effect of Water and Ethanol on the Formation of Inclusion Complex for a D-Limonene and Cyclodextrin System.- An Overview and Future Perspectives for Bioprocess Systems Engineering.- Screening of High Cellulase Producing Mutants and Culture of Cellulose Koji with Rice Straw and Stillage.- A Study of Ethanol Fermentation Using a Flocculent Strain of Zymomonas mobilis.- Pasteurization of Solid Food by Far Infrared Irradiation.- 12. Environmental Engineering.- Biogas Upgrading Using Membrane Gas Permeators.- Enzymatic Membrane Reactor for Cyanide Degradation in Food Industry Effluents.- Development of an Immobilized Microbial Process for Degrading Polyvinyl Alcohol in Wastewater.- Characteristics of Granular Methanogenic Sludge Grown on Different Substrates in an Upflow Anaerobic Sludge Blanket (UASB) Reactor.- Study on Scale-Up of UASB Reactors.- A Mathematical Model for the Anaerobic Degradation of 2-Ethylhexanoic Acid.- A Novel Bioreactor for the Biodegradation of Toxic Aromatic Solvents.- H2S Removal in a Photosynthetic Fed-Batch Reactor with Chlorobium thiosulfatophilum.- Relation Between Kinetic Parameters and BOD Load in Simulation of BOD and P Behaviors in a Sequencing Batch Reactor.- Biological Treatment of Wastewater Containing High Content of Phenol.- Kinetics of Nitrogen and BOD Removal in Biofilm Processes.- Study of Treatment of Wastewater Produced by Starch Alcohol with 12M3 Spilled Self-Sucking Fermenter.- The Effect of Aromatic Compounds on Trichloroethylene Degradation Using Pseudomonas Species.- Deodorization of Waste Gases in Packed Columns with Immobilized Activated Sludge Gel Beads.- Enhanced Removal Efficiency of Malodorous Gases in Pilot-Scale Peat Biofilter Inoculated with Thiobacillus thioparus DW44.- Changes of Trihalomethane Formation Potential in BAC Treatment.- Virus Behavior in Activated Sludge Culture.- Inhibitory Effect of Ionic Nitrogen Oxides on Methanogenesis of Methanol in Methanogenic Sludge.- Author Index.